Peter Parker's loving aunt who raised him after his parents' death. She is the moral center of his life and his motivation to be a better man.

A skilled master thief who has a complicated romantic relationship with Spider-Man. She loves the hero but often has little interest in the man behind the mask.

A brilliant scientist with four mechanical arms fused to his body. Once a mentor figure, his arrogance and accident turned him into one of Spider-Man's most dangerous foes.

Spider-Man's greatest enemy. A wealthy industrialist driven insane by his own experimental formula, he is obsessed with destroying Spider-Man physically and psychologically.

Peter Parker's first true love. Brilliant and kind, her tragic death at the hands of the Green Goblin changed Peter forever. In other universes, she becomes the hero Spider-Gwen.

The loud, cigar-smoking editor of the Daily Bugle who leads a crusade against Spider-Man, labeling him a 'mece.' Despite his gruff exterior, he is a man of integrity who protects his staff.

Peter Parker's primary love interest and eventual wife. Smart, independent, and supportive, she knows Peter's secret and grounds him.

A creative teeger from Brooklyn who gains spider powers and takes up the mantle. He brings a fresh perspective and unique powers like invisibility and venom blasts.

A teeger from Brooklyn who becomes Spider-Man in the Ultimate universe...

A brilliant but nerdy teeger who gains spider powers and learns to be a hero. Defined by his sense of responsibility and refusal to give up, he protects New York while struggling to pay rent.

A brilliant but nerdy teeger who gains spider powers and learns to be a hero...

The result of the bonding between Eddie Brock and an alien symbiote rejected by Peter Parker. Venom possesses all of Spider-Man's powers but with brute force and a twisted sense of justice.